COVID-19
REACT Observational Study of COVID-19
Year Started: 2020
SARS-CoV-2 has emerged as a global pandemic in 2020. There are no effective treatments and an effective vaccine has not been developed. Whilst many patients recover without need for hospitalisation, a small proportion go on to develop severe disease. A better understanding of the natural history of this disease will facilitate improved clinical management with the potential to identify options for intervention. This proposal is for the formal collection of characterisation data for patients tested for and/or admitted with a confirmed diagnosis of SARS-CoV-2 infection. This study responds to the need for a clear understanding of the natural history of this novel, emerging pandemic. Specifically phenotypic characterisation may enable application of personalised medical approaches, based on clinical course, to aid earlier identification of patients at risk of severe disease, and facilitate identification of potential targets for novel treatments. The collection and characterisation of these patients reflects a strong partnership between NHS and academic critical care and respiratory medicine in Southampton that acts as both a secondary and tertiary centre, with support from the digital experimental cancer medicine team at the University of Manchester.
- Access:
- Access restricted at present
- Type:
- Disease specific
- Status:
- In progress
- Consent restrictions:
- Commercial restriction, Xenograft restriction
Associated Data Type | Procurement Timeframe |
---|---|
Biomarker datasets | > 6 months |
Clinical records | > 6 months |
Followup records | > 6 months |
Freezer temperature logs | > 6 months |
Pathology records | > 6 months |
Physiological/biochemical measurements | > 6 months |
Quality indicators | > 6 months |
Treatment records | > 6 months |
Donor Ethnicity | > 6 months |
Male
Adult (> 40 years)
11 - 100 donors
Material Type | Extraction Procedure | Storage Temperature | Preservation Type | Macroscopic Assessment | % of Sample Set |
---|---|---|---|---|---|
Serum | -60°C to -85°C | Not applicable | 51 - 75% | ||
Plasma | -60°C to -85°C | Not applicable | 11 - 25% | ||
Whole blood | -60°C to -85°C | Not applicable | 0 - 10% | ||
Swab | -60°C to -85°C | Not applicable | 0 - 10% | ||
Saliva | -60°C to -85°C | Not applicable | 11 - 25% | ||
Peripheral blood mononuclear cells (PBMC) | Liquid nitrogen vapor phase | Not applicable | 0 - 10% | ||
Urine | -60°C to -85°C | Not applicable | 0 - 10% | ||
RNA | -60°C to -85°C | Not applicable | 0 - 10% |
Female
Adult (> 40 years)
11 - 100 donors
Material Type | Extraction Procedure | Storage Temperature | Preservation Type | Macroscopic Assessment | % of Sample Set |
---|---|---|---|---|---|
Serum | -60°C to -85°C | Not applicable | 51 - 75% | ||
Plasma | -60°C to -85°C | Not applicable | 11 - 25% | ||
Whole blood | -60°C to -85°C | Not applicable | 0 - 10% | ||
Swab | -60°C to -85°C | Not applicable | 0 - 10% | ||
Saliva | -60°C to -85°C | Not applicable | 11 - 25% | ||
Peripheral blood mononuclear cells (PBMC) | Liquid nitrogen vapor phase | Not applicable | 0 - 10% | ||
Urine | -60°C to -85°C | Not applicable | 0 - 10% | ||
RNA | -60°C to -85°C | Not applicable | 0 - 10% |
Fit and well (finding)
Isolation of peripheral blood mononuclear cells (PBMC) from whole blood donated by healthy volunteers for laboratory certification purposes
Year Started: 2017
Due to increasing demand for the validation of staff performing white blood cell isolation from whole blood, laboratory operators based in the University of Southampton and University Hospital Southampton NHS Foundation Trust must be certified by conducting the activities described in specific standard operating procedures (SOPs) for the separation of white blood cells from whole blood. In order to validate the laboratory operators for the purpose of certification, blood from healthy volunteers is required. The isolated white cells will undergo analysis to determine how many cells were isolated, if the cells are dead or alive and if the cells have been excessively stimulated during the procedure or not. If there are any cells left over after the analysis has been done, they will be destroyed. If the quantity and quality of the white blood cells falls within expected parameters, the operators performing the procedure will be validated and certified for that particular SOP.
- Access:
- Access restricted at present
- Type:
- Cohort
- Status:
- In progress
- Consent restrictions:
- Commercial restriction, Xenograft restriction
Associated Data Type | Procurement Timeframe |
---|---|
Biomarker datasets | > 6 months |
Clinical records | > 6 months |
Freezer temperature logs | > 6 months |
Physiological/biochemical measurements | > 6 months |
Quality indicators | > 6 months |
Donor Ethnicity | > 6 months |
Female
Adult (> 40 years)
11 - 100 donors
Material Type | Extraction Procedure | Storage Temperature | Preservation Type | Macroscopic Assessment | % of Sample Set |
---|---|---|---|---|---|
Whole blood | -60°C to -85°C | Not applicable | 100% | ||
Peripheral blood mononuclear cells (PBMC) | Liquid nitrogen vapor phase | Not applicable | 100% |
Male
Adult (> 40 years)
11 - 100 donors
Material Type | Extraction Procedure | Storage Temperature | Preservation Type | Macroscopic Assessment | % of Sample Set |
---|---|---|---|---|---|
Whole blood | -60°C to -85°C | Not applicable | 100% | ||
Peripheral blood mononuclear cells (PBMC) | Liquid nitrogen vapor phase | Not applicable | 100% |
Severe combined immunodeficiency due to absent interleukin-2 receptor (disorder)
Deep immuno-phenotyping to identify precision medicine targets in adult and paediatric patients with Combined Immunodeficiency
Year Started: 2016
Primary Immunodeficiencies (PIDs) are rare inherited diseases of the immune system that may lead to recurrent infections, malignancy or autoimmunity, all with a significantly impaired quality of life. There are currently over 200 different PIDs with approximately 250 different genes linked to these disorders. Through the NIHR/Wellcome Trust Southampton Clinical Research Facility for Experimental Medicine and Translational Medicine Laboratory, we will perform deep-phenotyping (detailed examination of possible pertinent findings of gene tests) of 20 patients with PID to gain additional understanding of the cause each patients’ rare immunological disease. This greater understanding will hopefully identify novel therapeutic targets and the initiation of personalised treatments. Previously at our centre we have shown that, by this approach, we can commence precision therapeutics for patients with PID that substantially reduced morbidity and mortality.
- Access:
- Access restricted at present
- Type:
- Disease specific
- Status:
- In progress
- Consent restrictions:
- Commercial restriction, Xenograft restriction
Associated Data Type | Procurement Timeframe |
---|---|
Biomarker datasets | > 6 months |
Clinical records | > 6 months |
Followup records | > 6 months |
Genomic datasets | > 6 months |
Pathology records | > 6 months |
Quality indicators | > 6 months |
Treatment records | > 6 months |
Donor Ethnicity | > 6 months |
Female
Adult (> 40 years)
11 - 100 donors
Material Type | Extraction Procedure | Storage Temperature | Preservation Type | Macroscopic Assessment | % of Sample Set |
---|---|---|---|---|---|
Whole blood | -60°C to -85°C | Not applicable | 100% | ||
Serum | -60°C to -85°C | Not applicable | 100% | ||
Plasma | -60°C to -85°C | Not applicable | 100% | ||
RNA | -60°C to -85°C | Not applicable | 100% | ||
Peripheral blood mononuclear cells (PBMC) | Liquid nitrogen vapor phase | Not applicable | 51 - 75% |
Male
Adult (> 40 years)
11 - 100 donors
Material Type | Extraction Procedure | Storage Temperature | Preservation Type | Macroscopic Assessment | % of Sample Set |
---|---|---|---|---|---|
Whole blood | -60°C to -85°C | Not applicable | 100% | ||
Serum | -60°C to -85°C | Not applicable | 100% | ||
Plasma | -60°C to -85°C | Not applicable | 100% | ||
RNA | -60°C to -85°C | Not applicable | 100% | ||
Peripheral blood mononuclear cells (PBMC) | Liquid nitrogen vapor phase | Not applicable | 51 - 75% |