Fit and well (finding)
Isolation of peripheral blood mononuclear cells (PBMC) from whole blood donated by healthy volunteers for laboratory certification purposes
Year Started: 2017
Due to increasing demand for the validation of staff performing white blood cell isolation from whole blood, laboratory operators based in the University of Southampton and University Hospital Southampton NHS Foundation Trust must be certified by conducting the activities described in specific standard operating procedures (SOPs) for the separation of white blood cells from whole blood. In order to validate the laboratory operators for the purpose of certification, blood from healthy volunteers is required. The isolated white cells will undergo analysis to determine how many cells were isolated, if the cells are dead or alive and if the cells have been excessively stimulated during the procedure or not. If there are any cells left over after the analysis has been done, they will be destroyed. If the quantity and quality of the white blood cells falls within expected parameters, the operators performing the procedure will be validated and certified for that particular SOP.
- Access:
- Access restricted at present
- Type:
- Cohort
- Status:
- In progress
- Consent restrictions:
- Commercial restriction, Xenograft restriction
Associated Data Type | Procurement Timeframe |
---|---|
Biomarker datasets | > 6 months |
Clinical records | > 6 months |
Freezer temperature logs | > 6 months |
Physiological/biochemical measurements | > 6 months |
Quality indicators | > 6 months |
Donor Ethnicity | > 6 months |
Male
Young adult (18 - 40 years)
11 - 100 donors
Material Type | Extraction Procedure | Storage Temperature | Preservation Type | Macroscopic Assessment | % of Sample Set |
---|---|---|---|---|---|
Whole blood | -60°C to -85°C | Not applicable | 100% | ||
Peripheral blood mononuclear cells (PBMC) | Liquid nitrogen vapor phase | Not applicable | 100% |
Male
Adult (> 40 years)
11 - 100 donors
Material Type | Extraction Procedure | Storage Temperature | Preservation Type | Macroscopic Assessment | % of Sample Set |
---|---|---|---|---|---|
Whole blood | -60°C to -85°C | Not applicable | 100% | ||
Peripheral blood mononuclear cells (PBMC) | Liquid nitrogen vapor phase | Not applicable | 100% |